Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

Irene Costantini; Giacomo Mazzamuto; Annunziatina Laurino; Erica Lazzeri; Leonardo Sacconi; Mattia Neri; Andrea Simonetto; Matteo Roffilli; Ludovico Silvestri; Francesco S. Pavone

doi:10.1117/12.2527096

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

Irene Costantini
, Giacomo Mazzamuto
, Annunziatina Laurino
, Erica Lazzeri
, Leonardo Sacconi
, Mattia Neri
, Andrea Simonetto
, Matteo Roffilli
, Ludovico Silvestri
, Francesco S. Pavone

LENS, University of Florence
LENS
University of Florence
Bioretics Srl

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › Contribution à une conférence › Revue par des pairs

1 Citation (Scopus)

Résumé

The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.

langue originale	Anglais
titre	Advances in Microscopic Imaging II
rédacteurs en chef	Emmanuel Beaurepaire, Francesco Saverio Pavone
Editeur	SPIE
ISBN (Electronique)	9781510628458
Les DOIs	https://doi.org/10.1117/12.2527096
état	Publié - 1 janv. 2019
Modification externe	Oui
Evénement	Advances in Microscopic Imaging II 2019 - Munich, Allemagne Durée: 26 juin 2019 → 27 juin 2019

Série de publications

Nom	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11076
ISSN (imprimé)	1605-7422

Une conférence

Une conférence	Advances in Microscopic Imaging II 2019
Pays/Territoire	Allemagne
La ville	Munich
période	26/06/19 → 27/06/19

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Costantini, I., Mazzamuto, G., Laurino, A., Lazzeri, E., Sacconi, L., Neri, M., Simonetto, A., Roffilli, M., Silvestri, L., & Pavone, F. S. (2019). Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method. Dans E. Beaurepaire, & F. S. Pavone (eds.), Advances in Microscopic Imaging II Article 110760B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol 11076). SPIE. https://doi.org/10.1117/12.2527096

@inproceedings{f2dfa43a1654470c866f3da967f55870,

title = "Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method",

abstract = "The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.",

keywords = "3D imaging, Brain, Clearing, Human, SWITCH, TDE",

author = "Irene Costantini and Giacomo Mazzamuto and Annunziatina Laurino and Erica Lazzeri and Leonardo Sacconi and Mattia Neri and Andrea Simonetto and Matteo Roffilli and Ludovico Silvestri and Pavone, \{Francesco S.\}",

note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Advances in Microscopic Imaging II 2019 ; Conference date: 26-06-2019 Through 27-06-2019",

year = "2019",

month = jan,

day = "1",

doi = "10.1117/12.2527096",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Costantini, I, Mazzamuto, G, Laurino, A, Lazzeri, E, Sacconi, L, Neri, M, Simonetto, A, Roffilli, M, Silvestri, L & Pavone, FS 2019, Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method. Dans E Beaurepaire & FS Pavone (eds), Advances in Microscopic Imaging II., 110760B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, VOL. 11076, SPIE, Advances in Microscopic Imaging II 2019, Munich, Allemagne, 26/06/19. https://doi.org/10.1117/12.2527096

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method. / Costantini, Irene; Mazzamuto, Giacomo; Laurino, Annunziatina et al.
Advances in Microscopic Imaging II. Ed. / Emmanuel Beaurepaire; Francesco Saverio Pavone. SPIE, 2019. 110760B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol 11076).

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › Contribution à une conférence › Revue par des pairs

TY - GEN

T1 - Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

AU - Costantini, Irene

AU - Mazzamuto, Giacomo

AU - Laurino, Annunziatina

AU - Lazzeri, Erica

AU - Sacconi, Leonardo

AU - Neri, Mattia

AU - Simonetto, Andrea

AU - Roffilli, Matteo

AU - Silvestri, Ludovico

AU - Pavone, Francesco S.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.

AB - The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.

KW - 3D imaging

KW - Brain

KW - Clearing

KW - Human

KW - SWITCH

KW - TDE

UR - https://www.scopus.com/pages/publications/85074301285

U2 - 10.1117/12.2527096

DO - 10.1117/12.2527096

M3 - Conference contribution

AN - SCOPUS:85074301285

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Advances in Microscopic Imaging II

A2 - Beaurepaire, Emmanuel

A2 - Pavone, Francesco Saverio

PB - SPIE

T2 - Advances in Microscopic Imaging II 2019

Y2 - 26 June 2019 through 27 June 2019

ER -

Costantini I, Mazzamuto G, Laurino A, Lazzeri E, Sacconi L, Neri M et al. Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method. Dans Beaurepaire E, Pavone FS, rédacteurs en chef, Advances in Microscopic Imaging II. SPIE. 2019. 110760B. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2527096

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

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SDG des Nations Unies

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