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Ultrafast conformational changes in biomolecules studied by time-resolved circular dichroism

  • Université Paris-Saclay

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Résumé

Circular dichroism (CD) is known to be a very sensitive probe of molecular conformation, and it is in particular widely used in biochemistry. Measuring the CD as a function of time is therefore very appealing to access information on the dynamics of conformational changes in molecules or biomolecules. We have implemented such a time-resolved experiments in two complementary configurations: a sub-picosecond pump-probe one and a microsecond detection of CD coupled to a T-jump experiment. We present two experiments based on these techniques: the ultrafast motion of the carbonyl group in the chromophore of the Photoactive Yellow Protein after photoexcitation and the dynamics of thermal denaturation in model peptides.

langue originaleAnglais
titreOptics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII
rédacteurs en chefGari Owen, Roberto Zamboni, Harbinder Rana, Attila A. Szep, Douglas Burgess, Francois Kajzar
EditeurSPIE
ISBN (Electronique)9781628418620
Les DOIs
étatPublié - 1 janv. 2015
Modification externeOui
EvénementOptics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII - Toulouse, France
Durée: 21 sept. 201523 sept. 2015

Série de publications

NomProceedings of SPIE - The International Society for Optical Engineering
Volume9652
ISSN (imprimé)0277-786X
ISSN (Electronique)1996-756X

Une conférence

Une conférenceOptics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII
Pays/TerritoireFrance
La villeToulouse
période21/09/1523/09/15

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