UV melting of G-quadruplexes

Jean Louis Mergny; Laurent Lacroix

doi:10.1002/0471142700.nc1701s37

UV melting of G-quadruplexes

Jean Louis Mergny
, Laurent Lacroix

CNRS/Museum National d'Histoire Naturelle/IRD/UPMC

Résultats de recherche: Contribution à un journal › Article de révision › Revue par des pairs

Résumé

Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (T_m values) and model-dependent thermodynamic parameters.

langue originale	Anglais
Pages (de - à)	17.1-1-17.1-15
journal	Current Protocols in Nucleic Acid Chemistry
Numéro de publication	SUPPL. 37
Les DOIs	https://doi.org/10.1002/0471142700.nc1701s37
état	Publié - 17 juin 2009
Modification externe	Oui

Accès au document

10.1002/0471142700.nc1701s37

Contient cette citation

@article{e8cb73196a1a46ecad4d2866c5502467,

title = "UV melting of G-quadruplexes",

abstract = "Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (Tm values) and model-dependent thermodynamic parameters.",

keywords = "Quartets, Thermal melting, Thermodynamics, van't Hoff analysis",

author = "Mergny, \{Jean Louis\} and Laurent Lacroix",

year = "2009",

month = jun,

day = "17",

doi = "10.1002/0471142700.nc1701s37",

language = "English",

pages = "17.1--1--17.1--15",

journal = "Current Protocols in Nucleic Acid Chemistry",

issn = "1934-9270",

number = "SUPPL. 37",

}

TY - JOUR

T1 - UV melting of G-quadruplexes

AU - Mergny, Jean Louis

AU - Lacroix, Laurent

PY - 2009/6/17

Y1 - 2009/6/17

N2 - Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (Tm values) and model-dependent thermodynamic parameters.

AB - Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (Tm values) and model-dependent thermodynamic parameters.

KW - Quartets

KW - Thermal melting

KW - Thermodynamics

KW - van't Hoff analysis

U2 - 10.1002/0471142700.nc1701s37

DO - 10.1002/0471142700.nc1701s37

M3 - Review article

C2 - 19488970

AN - SCOPUS:66949118904

SN - 1934-9270

SP - 17.1-1-17.1-15

JO - Current Protocols in Nucleic Acid Chemistry

JF - Current Protocols in Nucleic Acid Chemistry

IS - SUPPL. 37

ER -

UV melting of G-quadruplexes

Résumé

Accès au document

Empreinte digitale

Contient cette citation